a2780 cells  (European Collection of Authenticated Cell Cultures)

Journal: Scientific Reports

Article Title: Synthesis, spectral, thermal, and biological characterization of Se(IV) nanocomplexes derived from vitamin E and amino acid mixed ligands as a metal-drug model

doi: 10.1038/s41598-025-30281-1

Figure Lengend Snippet: The phase contrast image of HepG2 and A2780 cells upon treatment with Se-Vit E-Tyr(III) complex.

Article Snippet: HepG2 and A2780 cell lines were purchased from ATCC ( https://www.atcc.org/ ).

Techniques:

Journal: Molecular Medicine Reports

Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines

doi: 10.3892/mmr.2025.13611

Figure Lengend Snippet: Expression levels of miR-145 and miR-23b after transfection of ovarian cancer cells. A2780, SKOV-3 and OV-90 cells were transfected with miR-145, miR-23b, Mix, Sc or Control using Viafect reagent for 48 h at 37°C. RNU6 was used as the control to quantify mRNA levels. n=3 for A2780, SKOV and OV-90 cells. *P<0.05 and **P<0.01 vs. Control (Kruskal-Wallis test with Dunn's post hoc test). Data are presented as the mean ± SEM. miR, microRNA; Mix, combination of miR-145 and miR-23b; Sc, scrambled sequence; RNU6, small nuclear RNA U6.

Article Snippet: The A2780 cells originate from a human patient with primary EOC without chemotherapy treatment, and were obtained from the European Collection of Authenticated Cell Cultures.

Techniques: Expressing, Transfection, Control, Sequencing

Journal: Molecular Medicine Reports

Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines

doi: 10.3892/mmr.2025.13611

Figure Lengend Snippet: Effect of miR-145 and miR-23b co-transfection on the proliferation of EOC cells. Cell proliferation was assessed using Ki-67 immunofluorescence staining and EOC cell quantification was conducted using Ki-67 immunofluorescence images (red cells). Representative immunofluorescence images of Ki-67 (red) in (A) A2780, (C) SKOV-3 and (E) OV-90 cells. Magnification, ×400; scale bar, 50 µm. Semi-quantitative analysis of Ki-67 immunofluorescence in (B) A2780, (D) SKOV-3 and (F) OV-90 cells was conducted using Image Pro-Plus 6.2 computer software. Data for semi-quantification of fluorescence were normalized to the Control condition. n=4 (4–6 images per condition). Semi-quantitative analysis of Ki-67-positive EOC cells (red) in (G) A2780, (H) SKOV-3 and (I) OV-90 cells was carried out using the Fiji ImageJ program. n=4 (3–4 images per condition were analyzed; magnification, ×400). Conditions were normalized to the Control. *P<0.05, **P<0.01 and ***P<0.001 compared vs. Control (Kruskal-Wallis test with Dunn's post hoc test). Data are presented as the mean ± SEM. miR, microRNA; EOC, epithelial ovarian cancer; Mix, combination of miR-145 and miR-23b; Sc, scrambled sequence.

Article Snippet: The A2780 cells originate from a human patient with primary EOC without chemotherapy treatment, and were obtained from the European Collection of Authenticated Cell Cultures.

Techniques: Cotransfection, Immunofluorescence, Staining, Software, Fluorescence, Control, Sequencing

Journal: Molecular Medicine Reports

Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines

doi: 10.3892/mmr.2025.13611

Figure Lengend Snippet: Effect of miR-145 and miR-23b co-transfection on epithelial ovarian cancer cell migration. Migration was evaluated using Transwell inserts. Representative images of post-treatment migration assays in (A) A2780, (B) SKOV-3 and (C) OV-90 cells. Scale bar, 50 µm. Semi-quantitative analysis of migration data in (D) A2780, (E) SKOV-3 and (F) OV-90 cells. Changes are presented relative to the Control. n=3 (6–8 images per condition). *P<0.05, **P<0.01 and ***P<0.001 vs. Control (Kruskal-Wallis test followed by Dunn's post hoc test). a P<0.05 and aa P<0.01 vs. Mix (Kruskal-Wallis test followed by Dunn's post hoc test). Data are presented as the mean ± SEM. miR, microRNA; Mix, combination of miR-145 and miR-23b; Sc, scrambled sequence.

Article Snippet: The A2780 cells originate from a human patient with primary EOC without chemotherapy treatment, and were obtained from the European Collection of Authenticated Cell Cultures.

Techniques: Cotransfection, Migration, Control, Sequencing

Journal: Molecular Medicine Reports

Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines

doi: 10.3892/mmr.2025.13611

Figure Lengend Snippet: Effect of miR-145 and miR-23b co-transfection on epithelial ovarian cancer cell invasion. The invasion assay was carried out using Matrigel-coated Transwell inserts. Representative images of invasion assays following treatment with miRs at 37°C for 24 h in (A) A2780 and (E) OV-90 cells, and (C) 16 h in SKOV-3 cells. Scale bar, 50 µm. Semi-quantitative analysis of images of invasion data in (B) A2780, (D) SKOV-3 and (F) OV-90 cells. Data are presented as multiples of change relative to the Control. n=3 (10 images per condition). ***P<0.001 vs. Control condition (Kruskal-Wallis test followed by Dunn's post hoc test). Data are presented as the mean ± SEM. miR, microRNA; Mix, combination of miR-145 and miR-23b; Sc, scrambled sequence.

Article Snippet: The A2780 cells originate from a human patient with primary EOC without chemotherapy treatment, and were obtained from the European Collection of Authenticated Cell Cultures.

Techniques: Cotransfection, Invasion Assay, Control, Sequencing

Journal: Molecular Medicine Reports

Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines

doi: 10.3892/mmr.2025.13611

Figure Lengend Snippet: Western blot analysis of c-MYC in EOC cells. Representative images c-MYC protein expression (57–65 kDa) in (A) A2780, (C) SKOV-3 and (E) OV-90 cells. A total of 50 µg of protein (A2780 and OV-90 cells) and 80 µg protein (SKOV-3 cells) were loaded, and β-actin (42 kDa) was used as a loading control. Semi-quantitative analysis of the western blotting bands in EOC cells after transfection under different conditions in (B) A2780, (D) SKOV-3 and (F) OV-90 cells. Data were normalized to β-actin and are presented as the fold change compared with the Control. n=4. *P<0.05, **P<0.01 and ***P<0.001 vs. Control (Kruskal-Wallis test followed by Dunn's post hoc test). Data are presented as the mean ± SEM. miR, microRNA; EOC, epithelial ovarian cancer; Mix, combination of miR-145 and miR-23b; Sc, scrambled sequence; AU, arbitrary units.

Article Snippet: The A2780 cells originate from a human patient with primary EOC without chemotherapy treatment, and were obtained from the European Collection of Authenticated Cell Cultures.

Techniques: Western Blot, Expressing, Control, Transfection, Sequencing

Journal: Molecular Medicine Reports

Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines

doi: 10.3892/mmr.2025.13611

Figure Lengend Snippet: Western blot analysis of ZEB1 expression in EOC cells. Representative images of ZEB1 protein expression (125–200 kDa) in (A) A2780, (C) SKOV-3 and (E) OV-90 EOC cells. A total of 50 µg (A2780 and SKOV-3 cells) or 80 µg (OV-90 cells) of protein were loaded. β-actin (42 kDa) was used as a loading control. Semi-quantitative analysis of the western blotting bands in (B) A2780, (D) SKOV-3 and (F) OV-90 cells after transfection under different conditions. Values were normalized to β-actin and expressed as the fold change compared with the Control. n=4. *P<0.05, **P<0.01 and ***P<0.001 vs. Control (Kruskal-Wallis test followed by Dunn's post hoc test). Data are presented as the mean ± SEM. miR, microRNA; EOC, epithelial ovarian cancer; Mix, combination of miR-145 and miR-23b; Sc, scrambled sequence; AU, arbitrary units; ZEB1, zinc finger E-box binding homeobox 1.

Article Snippet: The A2780 cells originate from a human patient with primary EOC without chemotherapy treatment, and were obtained from the European Collection of Authenticated Cell Cultures.

Techniques: Western Blot, Expressing, Control, Transfection, Sequencing, Binding Assay

Journal: Molecular Medicine Reports

Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines

doi: 10.3892/mmr.2025.13611

Figure Lengend Snippet: Western blot analysis of ABCB1 in A2780 and SKOV-3 cells. Representative images of ABCB1 (130–180 kDa) protein expression in (A) A2780 and (C) SKOV-3 cells. A total of 80 µg protein was loaded. β-actin (42 kDa) was used as the loading control. Semi-quantitative analysis of western blot bands in (B) A2780 and (D) SKOV-3 cells post-transfection under the different conditions. Values were normalized to β-actin and the Control. n=4. *P<0.05, **P<0.01 and ***P<0.001 vs. Control (Kruskal-Wallis test followed by Dunn's post hoc test). Data are presented as the mean ± SEM. miR, microRNA; Mix, combination of miR-145 and miR-23b; Sc, scrambled sequence; AU, arbitrary units; ABCB1, ATP binding cassette subfamily B1.

Article Snippet: The A2780 cells originate from a human patient with primary EOC without chemotherapy treatment, and were obtained from the European Collection of Authenticated Cell Cultures.

Techniques: Western Blot, Expressing, Control, Transfection, Sequencing, Binding Assay